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Haematology & Transfusion » Specimens, requests, results » Haematology tests

Last update to this site: 20.8.2014
    

Lab Tests OnlineUK
HaematologyHaematology tests

For information on those tests not listed please contact technical staff in the department who will be happy to advise or to pass you on to a consultant if necessary.

Volumes are for adults' samples - smaller tubes are available for paediatric samples.

ADAMTS13Referred to Haemostasis Research Unit, UCL Haematology Dept, 51 Chenies Mews, London WC1E 6HX.

Haematology (coagulation)

Only available after discussion with a consultant haematologist.

Blue cap tube (trisodium citrate)3 x 1.8 mL Na-citrate blood. If UK TTP Registry required, Gold cap tube (serum with separator gel)5 mL clotted blood. Mix samples immediately by gentle inversion and store at room temperature. Samples must reach the laboratory as soon as possible after being taken - centrifugation and separation within 4 hours of being taken is required.

Referal laboratory quotes turnaround time as 2 weeks for ADAMTS13 activity, and 3 weeks for ADAMTS13 antibody assay, although in practice may be quicker.

See report.

Antithrombin activity

See thrombophilia screen.

APC resistance

See thrombophilia screen.

APTT inhibitor screen (50:50 mix)

Haematology (coagulation)

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Turnaround: 1 working day

Interpretive report

APTT ratio (activated partial thromboplastin time ratio)

Haematology (coagulation)

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Routine test, available 24/7Turnaround: 1 working day

Reference range:

0.8 - 1.2

APTT ratio - Therapeutic Heparin Ratio

Haematology (coagulation)

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Routine test, available 24/7Turnaround: 1 working day

Reference range:

2.0 – 2.5

B12 (vitamin B12) (click here for folate)

haematology (analysed in biochemistry) /Biochemistry

Gold cap tube (serum with separator gel)5 mL clotted blood

Turnaround: 24h (weekdays)

Reference range:

197 - 866 pg/mL

B12 and folate assays are done in Biochemistry but interpreted in Haematology - please contact Biochemistry for technical/analytical queries, Haematology for clinical interpretation and advice.
BCR-ABLReferred to HMDC Laboratory, Kings College Hospital, London SE5 9RS

Haematology

Lavender/black cap tube (EDTA)5 x 4 mL EDTA blood

Referred by Haematology Department by agreement of consultant haematologist.

Turnaround: 2-4 weeks

Interpretive report

Bleeding Time

Haematology

Available only after referral to consultant haematologist.

Test done on patient, not sample.

Turnaround time: 2 weeks

Reference range not applicable - see report.

Bone Marrow Aspiration

Haematology

Requires referral to consultant haematologist.

Turnaround time: <1 week (1+ week for trephine)

Interpretive clinical report

CD4/8 counts  

Immunohaematology test - click here

Chimerism studiesReferred to HMDC Laboratory, Kings College Hospital, London SE5 9RS

Haematology

Lavender/black cap tube (EDTA)5 x 4 mL EDTA blood

Referred by Haematology Department by agreement of consultant haematologist.

Turnaround: 2-4 weeks

Interpretive report

Chromosome studies for haematological investigation

Haematology

Lavender/black cap tube (EDTA)10 ml EDTA blood. Discuss with consultant haematologist before collection.

Turnaround time: 2 weeks

Interpretive report

Coagulation factor assays

Haematology (coagulation)

Only available after discussion with consultant haematologist.

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood, take with minimal stasis, must be filled to the line.

Please contact laboratory before collection for guidance on volume of blood required for factor in question. Send to laboratory as soon as possible.

  Reference range:
Factor II 79 - 150%
Factor V 62 - 139%
Factor VII
          Must be performed on a fresh specimen
50 - 150%

Factor IX

65 - 150%

Factor X

75 - 131%

Factor XI

65 - 150%

Factor XII

50 - 150%

Factor XIII screen
          2 tubes required  
          Only performed Monday to Thursday  
          Must arrive in the lab before 12 noon  
          TAT if prearranged 48hrs, otherwise 1 month
Reference range: See report.

Turnaround time: 1 month unless notified as urgent. Urgent results available within a few hours.

Coagulation screen

(INR and APTT ratio)

Haematology (coagulation) 

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Routine test, available 24/7Turnaround time: 1 working day (2 hours if identified as urgent).

Reference ranges:

INR: 0.8 - 1.2

APTT ratio: 0.8 - 1.2

D-dimers (fibrin degradation products, FDP)

Haematology (PRH) 

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Routine test, available 24/7Turnaround time: 1 working day (2 hours if identified as urgent).

Reference range:

<224 ng/mL

Unable to report on samples from pregnant patients or patients on anticoagulant therapy.

Erythrocyte sedimentation rate (ESR)

Haematology  

Lavender/black cap tube (EDTA)4 mL EDTA blood

Turnaround time: 1 working day 

Reference range:

0 to 15 mm in 1 hr

EMA test for red cell membrane abnormalities (e.g hereditary spherocytosis)Referred to IBGRL, NHSBT, North Bristol Park, Filton, Bristol BS34 7QG

Haematology

Lavender/black cap tube (EDTA)4 mL EDTA blood (1 mL minimum)

Only available after by prior arrangement with haematology staff. Samples to be taken in the morning to allow sufficent time for posting. Blood should also be taken from the affected parent as a positive control.

Turnaround time: 2 days

See report.

Factor V Leiden 

See thrombophilia screen.

Fibrinogen

Haematology (coagulation) 

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Routine test, available 24/7Turnaround time: 1 working day

Reference range:

2.0 - 4.0 g/L

Folate (click here for B12)

haematology (analysed in biochemistry) /Biochemistry

Gold cap tube (serum with separator gel)5 mL clotted blood

Turnaround: 24h (weekdays)

Reference range:

4.6 - 18.7 ng/mL

B12 and folate assays are done in Biochemistry but interpreted in Haematology - please contact Biochemistry for technical/analytical queries, Haematology for clinical interpretation and advice.

Full Blood Count (FBC)

Haematology 

Following guidance form the Pathology Harmony group and the DOH we have changed the units that Haemoglobin and MCHC are reported. From 9am on 25/3/13 results are reported in g/L rather than g/dL.

Lavender/black cap tube (EDTA)4 mL EDTA blood

Erroneous results can arise from aged samples (elevated mean cell volume), and contaminated samples (from drip lines).

Please send separate sample if requesting with Biochemistry EDTA tests (e.g. HbA1c)

Turnaround time: 1 working day (2 hours if identified as urgent).

Reference ranges: see below

 
 
Hb
g/L
RBC
x 1012/L
HCT
MCV
fL
MCH
pg
MCHC
g/L
Infant
149-237
3.7-6.5
0.47-0.75
100-135
32-39
310-360
2 weeks
134-198
3.9-5.9
0.41-0.65
84-120
30-35
2 months
111-141
3.1-4.3
0.28-0.42
68-82
29-32
6 years
113-141
3.9-5.3
0.31-0.41
68-87
27-32
12 years
115-155
4.0-5.2
0.35-0.45
77-95
25-32
adult female
115-165
3.8-5.8
0.37-0.47
76-100
27-32
adult male
135-180
4.5-6.5
0.40-0.54
 
 
WBC
x109/L
neutrophils 
x109/L
lymphocytes 
x109/L
monocytes 
x109/L
eosinophils 
x109/L
basophils 
x109/L
Infant
10.0-26.0
2.7-14.4
2.0-7.3
0.01-1.9
0-0.84
0-0.1
2 weeks
6.0-20.0
1.8-5.4
3.0-13.5
0.1-1.7
0-0.8
2 months
6.0-17.5
1.2-7.5
4.0-13.5
0.2-1.5
0-0.8
6 years
5.0-17.0
1.5-8.5
1.5-9.5
0.2-1.2
0-0.8
12 years
4.5-14.5
1.5-8.0
1.5-7.0
0.2-1.0
0-0.6
adult
4.0-11.0
2.0-7.5
1.3-3.5
0.2-0.8
0-0.4
Platelets 
150 - 450 x109/L
Erroneous results can arise from partially clotted samples,and in vitro anticoagulant effects.
Reticulocytes
click here
 
ESR
click here
 

Glandular Fever screen (Paul Bunnell, infectious mononucleosis, EBV)

Haematology 

Gold cap tube (serum with separator gel)5 mL clotted or Lavender/black cap tube (EDTA)4 mL EDTA blood (can be done on sample sent for FBC).

Reports show that around 10% of adults and 50% of young children can have a negative IM screen despite showing many typical clinical symptoms.

Turnaround time: 1 working day

Reference ranges not applicable - see report.

Glucose-6-phosphate dehydrogenase screen (G6PD)

Haematology (RSCH)

Lavender/black cap tube (EDTA)4 mL EDTA blood

Falsely high G6PD results may be seen after a haemolytic crisis due to increased reticulocytes which have higher G6PD levels than mature red cells.

Turnaround time: 1 week 

Reference range:

146 - 376 IU per 1012 RBC

Haemoglobinopathy screen (includes HbA2, HbF quantitation)

Haematology 

Lavender/black cap tube (EDTA)4 mL EDTA blood

For investigation of haemoglobinopathies e.g HbS,HbC, beta thalassaemia

Turnaround time: 3 working days (unless Hb variant found).

Reference ranges:

Haemoglobin HbA2: 2.0 - 3.2 %

Haemoglobin F (adults): ≤ 1.0 %

Samples are referred by Haematology Department after initial investigation for confirmation of haemoglobinopathy / thalassaemia by HPLC or molecular analysis. Referred to Red Cell Protein Laboratory, Haematology Department, King's College Hospital, London SE5 9RS, or National Haemoglobinopathy Reference Centre, Haematology Laboratory, John Radcliffe Hospital, Headington, Oxford OX3 9DUThis requires 2 x 4 mL EDTA blood.

Turnaround time: 4-6 weeks

Haemosiderin

Haematology

(test done by Cytology)

20 mL early morning urine.

Discuss with consultant haematologist before collection.

Turnaround time: 2 working days        

Interpretive clinical report

Heinz bodies

Haematology

Lavender/black cap tube (EDTA)4 mL EDTA blood

Turnaround time: 2 working days

Reference ranges not applicable - see report.

Heparin Assay (Anti-Xa)

Haematology

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

For monitoring LMW heparin. It is essential to state the type of heparin.

Blood should be taken 4-6 hours after administration of heparin.  This test can only be added to a request within 4 hours of sample collection.

Reference ranges:

Daltaparin (Fragmin)

Enoxaparin (Clexane)

Tinzaparin (Innohep)

Therapeutic range: 0.4 - 1.0 iu/mL

Prophylaxis range: 0.1-0.3 iu/mL

Danaparoid (Orgaran) Maintenance range: 0.5-0.8 U/mL

Turnaround time: the laboratory will try to turn around within 1 day if urgent, although there may sometimes be a delay as this is not a routine test.

Heparin Induced Thrombocytopenia test (urgent) Referred to Guy's & St. Thomas' Haemostasis Laboratory, 4th floor North Wing, St Thomas' Hospital, London SE1 9RT

Haematology

Blue cap tube (trisodium citrate)3 x 1.8ml Na-citrate and Gold cap tube (serum with separator gel)1x 5 mL clotted blood.

Referred by Haematology Department by agreement of consultant haematologist.

Turnaround time: within 1 week (urgent)

Interpretive report

HLA-B27

Immunohaematology test - click here

Immunohaematology Tests

Click here for more immunohaematology test information.

INR

Haematology (coagulation) 

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Routine test, available 24/7Turnaround time: 1 working day

Reference range:

0.8 - 1.2

JAK2 mutationReferred to HMDC Laboratory, Kings College Hospital, London SE5 9RS

Haematology (coagulation)

Lavender/black cap tube (EDTA)2 x 4 mL EDTA blood

Referred by Haematology Department by agreement of consultant haematologist.

Turnaround: 2-4 weeks

Interpretive clinical report.

Leukaemia immunophenotyping

Immunohaematology test - click here

Lupus anticoagulant

Haematology (coagulation)

Blue cap tube (trisodium citrate)2 x 1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

May be requested as part of thrombophilia screen, or as a separate test as in neurological or autoimmune investigations or recurrent miscarriage. May be affected by presence of heparin in levels greater than 1U/mL.

See Turnaround time: 1 month

Reference range:

<1.20

Lymphocyte subsets (B, T, NK cell counts)

Immunohaematology test - click here

Malarial parasites

Haematology 

Lavender/black cap tube (EDTA)4 mL EDTA blood

Turnaround time: same day

Confirmation of malaria sub type - 4 mL EDTA blood and slides referred by laboratory. Referred to Malaria Reference Laboratory, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HTTurnaround time: 1 week

Plasma viscosity

Haematology

Lavender/black cap tube (EDTA)4 mL EDTA blood

Turnaround time: 5 days unless specified as urgent.

Reference range:

1.50 - 1.72 cP

Platelets

see FBC - click here

Platelet Aggregation

Haematology

Special collection bottles.

Available only after discussion with consultant haematologist.

Reference ranges not applicable - see report.

Platelet Function Studies 

Haematology (RSCH)

Blue cap tube (trisodium citrate)2 x 1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Available only after discussion with consultant haematologist.

Turnaround time: same day

Reference range not applicable - see report.

Protein C

See thrombophilia screen

Protein S

See thrombophilia screen

Prothrombin gene mutation

See thrombophilia screen.

PT inhibitor screen

Haematology (RSCH)

Blue cap tube (trisodium citrate)2 x 1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Turnaround time: 1 working day

Interpretive report

Pyruvate kinase, red cellReferred to Haematology Dept, Kings College Hospital, London SE5 9RS

Haematology (RSCH)

Lavender/black cap tube (EDTA)4 ml EDTA (adult), 1 ml EDTA (paediatric)

Discuss with haematology laboratory.

Turnaround time: 2 weeks

Reference range:

11 - 19 IU/g Hb

Red cell mass/plasma volume estimation

Haematology with medical physics

Done at RSCH

Available only after discussion with consultant haematologist.

Red cell osmotic fragility

No longer available - please see EMA test for red cell membrane studies.

Reticulocytes

Haematology 

Lavender/black cap tube (EDTA)4 mL EDTA blood (can use FBC sample)

Turnaround time: 1 working day

Reference range:

Adult: 0.2 - 2.0%, 50 -100 x109/L

Infant: 2.0 - 6.0%, 74 -390 x109/L

Schilling Test

Haematology with medical physics

Available only after discussion with consultant haematologist. .

Sickle Cell screen

Haematology

Lavender/black cap tube (EDTA)4 mL EDTA blood

Turnaround time: 1 working day

T lymphocyte subsets

Immunohaematology test - click here

Thalassaemia screen

see see Haemoglobinopathy screen

Therapeutic Heparin Ratio

see APTT ratio - Therapeutic Heparin Ratio

Thrombin time

Haematology (coagulation)

Blue cap tube (trisodium citrate)1.8 mL Na-citrate blood

Take with minimal stasis, must be filled to the line.

Turnaround time: 1 working day

Reference range:

12 - 16.6 seconds

Thrombophilia screen

Haematology (coagulation)

General screen: Blue cap tube (trisodium citrate)4 x 1.8 mL Na- citrate blood taken with minimal stasis, filled to the line.

Best done 4 weeks after stopping warfarin, not at presentation or in non-steady state. BSUH guidelines suggest screen including FBC, coagulation screen,  thrombophilia screen including Factor V Leiden and prothrombin gene mutation, cardiolipin antibody & antinuclear antibody is carried out on patients less than 45 years of age who have an episode of venous thromboembolism.

Requests for individual tests (i.e. not as part of screen) should be discussed with the laboratory before submitting sample.

  Reference range:

Antithrombin activity

83 - 128%

APC resistance

>2.2

Lupus anticoagulant

<1.20

Protein C

70 - 140%

Protein S

male: 74 - 146%

female: 55 - 124%

Turnaround time: 1 month

Factor V Leiden and Prothrombin gene mutation

May be available after discussion with a consultant haematologist.

Lavender/black cap tube (EDTA)4 mL EDTA blood

Turnaround time: 1 month

von Willebrand screening

Haematology (coagulation)

Blue cap tube (trisodium citrate)2 x 1.8 mL Na-citrate blood, take with minimal stasis, must be filled to the line.

Available only after discussion with consultant haematologist.

 
Reference range:
(from 23/12/11)

Factor VIIIc

50 - 150 u/dL

VW activity 

Blood group O: 40 - 126%
Blood groups A, B, AB: 49 - 163 %

VW antigen

Blood group O: 42 - 141%
Blood groups A, B, AB: 66 - 176 %

Turnaround time: 1 month

See report.

White Cell Enzymes

Now being handled by Biochemistry - click here.

 

    

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